) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. This can increase throughput and efficiency, thus saving time and space in the lab. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Expt#3 . This unlocks the transport slide. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 6:30 . This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. After 5, 7, 11, and 13 days. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. Transfer of an assay to 96-well microplate technology increases throughput significantly. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. the appropriate well of the microplate. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. #T-0254) Lysozyme (Sigma cat. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 05. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Molecular Devices SpectraMax 384 Plus Microplate Reader. *Simple analysis and optimization. 0 OD at. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). It measures the optical density (OD) of samples in both 96- and 384-well. 5 mm. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. 384 Awareness Technology ChroMate BioTek Synergy Mx. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. iD5. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. i3x. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. SpectraMax iD5. High tolerance for contaminants. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 96-well microplate at 200 µL/well. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. M3-M5e_manual. The VersaMax reads 96-well microplates. When reading at wavelengths. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. 03 pM 384-well: 0. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 96-well solid bottom, clear polystyrene microplates (Greiner cat. In simplest terms, a plate reader measures and. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. absorbance, fluorescence, and luminescence with the added functionality of. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Add 150 µL reagent to each well. Cells were loaded with 2 µM Fluo-4 for one hour. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Abstract and Figures. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. 76 L x 85. LVF Monochromator™ : Excitation and Emission. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. for ValitaTiter Plus standards read on Molecular Devices readers. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. A preconfigured. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. With optimized reagents, option to operate the. 3. Optical System: High energy xenon flash lamp. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. 1-color 96-well . Align the two slots on the bottle holder with the two pegs on the rail. analysis software. 98 VMax R2 = 0. Five-second read per plate. With various on-board features and capabilities, the ThermoMax is. The eight-channel system delivers both high precision and speed when reading 96-well microplates for endpoint measurements, kinetic assays, and spectral scans. M5. 69: 85. Documentation:1. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. 2 OD at 200 nm. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 100 fmol 384-well (75 µL) 0. 3. g. Multi-Mode Microplate Reader for . Make sure to Data 96孔微孔板的数据对比. SOP Title: Use and. Microplate Spectrophotometers. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Molecular Devices M5 Plate Reader Plate Calibration Form . When reading at wavelengths. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. #12-639) White 96- and 384-well microplates (Greiner cat. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Page: 1/4. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. #W50-100) SpectraMax® ABS Plus Microplate Reader. ***Figure 1. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. i3x Multi-Mode Microplate Reader. Materials. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. explore more. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. Related Products: Low Binding Plates. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. #M5e) Methods Microplate reader compatibility test. Photometric and kinetic analysis mode. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Top or bottom detection is available with a simple click of a button. 300 µL of Detergent Compatible Bradford reagent was added to each well. The procedure enables direct translation of any published equation. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. New SpectraMax iD5 Multi-mode Microplate Reader. Both readers were heated to 37ºC prior to setting up the assay. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. reagent basin for ease of pipetting into. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. In . The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. The system provides automation for up to 50 microplates for easy walkaway automation. Compare specifications and request a quote from leading suppliers. SpectraDrop High-Throughput Screening (HTS) Kit. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Whatever your needs, you’ll find a microplate reader that can meet them. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The reader adds up to three transfers of assay reagents from a 96- or 384. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. It measures the optical density (OD) of samples in both 96- and 384-well. Making it possible to perform kinetic analysis of solid. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Transfer of an assay to 96-well microplate technology increases throughput significantly. 96-well: 0. Affordable multi-mode microplate reader with . 96- or 384-well microplate compatibility. Microplates . Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Microplate reader assays requiring infrared (IR) or red-shifted. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 14: Average %CV. Learn more about how absorbance is measured, and some key applications that utilize. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Filters: Excitation and emission slides for 4 filters each. It also integrates with the powerful SoftMax Pro Software for. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. white 96 well plate and 75. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. Please refer to the Obsolete Microplate Reader Models for more information. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. the iD5 reader’s filter system. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Microplates . Microplate readers from Molecular Devices are designed to provide consistent performance for many years. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. validation, maintenance, and repair documentation. Standard SBS 96 and 384 well plates (128. book Page 9 Wednesday, July 7, 2010 2:09 PM. Reactions catalyzed by firefly and *Renilla luciferases. 1 mL/well of media. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Microplate luminescent reader assay for ERK activity. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. Description. Industry standard, 96-well plates are. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 2 2600A ValitaTiter. Plate readers, also known as microplate readers or microplate photometers,. 020 fmol 0. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Multimode Reader Comparison Chart. #1402-9700) 384-well low volume solid black microplate (Corning cat. #ID3-STD) L-Tryptophan (Sigma cat. The Multiskan Sky microplate reader is an absorbance plate reader. Molecular Devices gmbH Tel. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. Detergent Compatible Bradford assay. Application Area: Spectrophotometer for molecular biology. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. An industry-renowned instrument for monitoring GPCRs and ion channels. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. Brand: MOLECULAR DEVICES. Published Dec 16, 2020 | Updated Dec 16, 2020. Suggested plates are black walled. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. 6. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. 14: Average %CV. 8. fluorescence microplate reader from Molecular Devices. M5. 10. adjust the reader. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. Timothy Bolus, MPM, MBA, Compliance Program Manager. Simple, economical . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 2 OD at 200 nm; this decreased to 0. Total volume (except deep. Reconstitution of reagents. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Or scan up to 384 wells at any time to determine the best assay conditions. Molecular Devices' microplate readers. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. personalized as your research. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The screw remains inside the instrument. July 17, 2023. For more sample throughput, the system canRating: 3. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Triplicates of each BSA concentration were made for this assay plate. 92: 88. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. If the best wavelengths are unknown, the Gemini EM reader will find. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. 01. book Page 9 Wednesday, July 7, 2010 2:09 PM. Vrla used the device to quantify the concentration of a small molecule produced by. 4 Pages. F3/F5 Multi-Mode Microplate Readers. Get. (FP) configured microplate readers. Four wash/rinse bottles are included and configurable for both 96- and 384. g. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. the iD5 reader’s filter system. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. You can add your own protocols. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. System setup and calibration are controlled from within SoftMax Pro Software. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. We would like to show you a description here but the site won’t allow us. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Step 1: Turn on your microplate reader. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Cross-talk < 0. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. 1536-well: 0. 48 W x 12. Well scanning With well scanning, multiple points within each well can be read, providing a high. Features. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. Other technologies such as imaging. #L6876) • Guanidine hydrochloride (Sigma cat. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Retrieving. measurements in compatible Molecular Devices® microplate readers. With various on-board features and. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. section, select plate section titled. Step 2: Launch SoftMax Pro Software. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 50 racks/case for 384-well (16 channel pipettor head. The concentration of total free thiols in the protein. Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. #ID3-STD) • L-Tryptophan (Sigma cat. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. We would like to show you a description here but the site won’t allow us. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. #SpectraMax L Config) Methods. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. 2 mm x 86 mm) Height:. The system is operated through the industry-leading SoftMax® Pro. Detection modalities are shown in Table 1-1. 0 OD Photometric resolution: 0. 96-well microplate; Immunoassay analyzer;VersaMax price. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. Read More. Microscopy. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). 5°C at 37°C. Softmax Pro GxP Software. Detection by Low noise PMT with simultaneous digital and analog modes. 03 pM 384-well: 0. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. to each well. explore more. microplate reader (Danaher Inc) Danaher Inc is a verified supplier Danaher Inc manufactures this product . Table 1: Categories of heating element design. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 7. The Molecular Devices Versamax Microplate Reader INCLUDED:. The OptiMax by Molecular Devices is a tunable microplate reader. (Figure 1). AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Endpoint, kinetic, spectrum. The device was able to achieve 3σ limits of detection of ca. Growth medium was then replaced with fatty acid uptake solution. For more sample throughput, the system can well. Materials. We would like to show you a description here but the site won’t allow us. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup.